塑料成分DEHP损伤胃肠促进炎症 | 热心肠日报

通过斑马鱼胃肠系统微生物组-宿主关系的评价，揭示适应性免疫是邻苯二甲酸酯（DEHP）的靶标

10.1021/acs.est.0c00628

04-07, Article

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To improve physical characteristics of plastics such as flexibility and durability, producers enrich materials with phthalates such as di-2-(ethylhexyl) phthalate (DEHP). DEHP is a high production volume chemical associated with metabolic and immune disruption in animals and humans. To reveal mechanisms implicated in phthalate-related disruption in the gastrointestinal system, male and female zebrafish were fed DEHP (3 ppm) daily for two months. At the transcriptome level, DEHP significantly upregulated gene networks in the intestine associated with helper T cells (Th1, Th2 and Th17) specific pathways. The activation of gene networks associated with adaptive immunity were linked to the suppression of networks for tight junction, gap junctional intercellular communication, and transmembrane transporters, all of which are precursors for impaired gut integrity and performance. On a class level, DEHP exposure increased Bacteroidia and Gammaproteobacteria and decreased Verrucomicrobiae in both the male and female gastrointestinal system. Further, in males there was a relative increase in Fusobacteriia, Betaproteobacteria and a relative decrease in Saccharibacteria. Predictive algorithms revealed the functional shift in the microbiome community, and the metabolites they produce, act to modulate intestinal adaptive immunity. This finding suggests that the gut microbiota may contribute to the adverse effects of DEHP on the host by altering metabolites sensed by both intestinal and immune Th cells. Our results suggest that the microbiome-gut-immune axis can be modified by DEHP and emphasizes the value of multi-omics approaches to study microbiome-host interactions following chemical perturbations.

First Authors:
Ondrej Adamovsky

Correspondence Authors:
Joseph H Bisesi Jr,Christopher J Martyniuk

All Authors:
Ondrej Adamovsky,Amanda N Buerger,Hana Vespalcova,Shahadur R Sohag,Amy T Hanlon,Pamela E Ginn,Serena L Craft,Stanislav Smatana,Eva Budinska,Maria Persico,Joseph H Bisesi Jr,Christopher J Martyniuk