橙汁摄入及其植物性微量营养素的显著抗氧化作用：轻度高胆固醇血症男性初步随机交叉试验

Clin Nutr. 2015 Dec;34(6):1093-100.

Marked antioxidant effect of orange juice intake and its phytomicronutrients in a preliminary randomized cross-over trial on mild hypercholesterolemic men.

Constans J, Bennetau-Pelissero C, Martin JF, Rock E, Mazur A, Bedel A, Morand C, Bérard AM

CHU - Université de Bordeaux, ERU «Facteurs de risque vasculaires», case 49, 146 rue Léo Saignat, F-33076 Bordeaux, France.

Université de Bordeaux, Physiopathologie de la plasticité neuronale, U862, F-33077 Bordeaux, France.

INSERM, Physiopathologie de la plasticité neuronale, U862, F-33077 Bordeaux, France.

INRA, UMR 1019, UNH, CRNH Auvergne, F-63000 Clermont-Ferrand, France.

Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, BP 10448, F-63000 Clermont-Ferrand, France.

BACKGROUND & AIMS: Blond orange juice is the most consumed fruit juice in the world. It is a source of hesperidin, a bioavailable flavonoid reported to exhibit potential vascular protective actions. However, the specific impact on vascular function of Citrus phytomicronutrients, is unknown. For the first time, we investigated the effects of blond orange juice compared with a control beverage mimicking the composition of orange juice (including Vitamin C but no phytomicronutrients), on antioxidant markers, cardiovascular risk factors and endothelial function.

METHODS: Twenty five male volunteers with two cardiovascular risk factors (age over 50 years and LDL-cholesterol between 130 and 190 mg/L) were enrolled in a randomized cross-over study. They received 3 times daily 200 mL of either blond orange juice or control beverage for 4 weeks, spaced by a 5-week wash-out. Endothelial function (flow mediated dilatation and plasma markers), oxidative status, lipid profile and inflammatory markers were assessed.

RESULTS: Daily intakes of orange juice significantly led to a marked antioxidant effect which was correlated to hesperetin plasma levels and related with a decrease in reactive oxygen species. A tendency towards reduction of endothelial dysfunction and modest increase in plasma apoA-I concentration were also observed. This allows further experiments demonstrating the specific effect of phytomicronutrients from orange juice.

CONCLUSIONS: These findings suggest that daily intake of nutritionally relevant dose of blond orange juice may contribute for a significant antioxidant effect through the phytochemicals contained in. Orange juice may be associated to other healthy foods to achieve a significant effect on the vascular function. This study is recorded in ClinicalTrials.com as NCT00539916.

KEYWORDS: Flow mediated dilatation; Hesperidin; Human cross-over study; Orange juice; Oxidative stress

PMID: 25865966

DOI: 10.1016/j.clnu.2014.12.016