类生物膜微菌落可显著提高副干酪乳杆菌的益生功能 | 热心肠日报

果胶钙小球包裹副干酪乳杆菌构成类生物膜结构，增强菌群在肠道的益生功能

10.1038/s41522-020-00159-3

10-28, Article

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In this study, we show that calcium pectinate beads (CPB) allow the formation of 20 µm spherical microcolonies of the probiotic bacteria Lacticaseibacillus paracasei (formerly designated as Lactobacillus paracasei) ATCC334 with a high cell density, reaching more than 10 log (CFU/g). The bacteria within these microcolonies are well structured and adhere to a three-dimensional network made of calcium-pectinate through the synthesis of extracellular polymeric substances (EPS) and thus display a biofilm-like phenotype, an attractive property for their use as probiotics. During bacterial development in the CPB, a coalescence phenomenon arises between neighboring microcolonies accompanied by their peripheral spatialization within the bead. Moreover, the cells of L. paracasei ATCC334 encased in these pectinate beads exhibit increased resistance to acidic stress (pH 1.5), osmotic stress (4.5 M NaCl), the freeze-drying process and combined stresses, simulating the harsh conditions encountered in the gastrointestinal (GI) tract. In vivo, the oral administration of CPB-formulated L. paracasei ATCC334 in mice demonstrated that biofilm-like microcolonies are successfully released from the CPB matrix in the colonic environment. In addition, these CPB-formulated probiotic bacteria display the ability to reduce the severity of a DSS-induced colitis mouse model, with a decrease in colonic mucosal injuries, less inflammation, and reduced weight loss compared to DSS control mice. To conclude, this work paves the way for a new form of probiotic administration in the form of biofilm-like microcolonies with enhanced functionalities.

First Authors:
Arnaud Heumann

Correspondence Authors:
Ali Assifaoui,Aurélie Rieu

All Authors:
Arnaud Heumann,Ali Assifaoui,David Da Silva Barreira,Charles Thomas,Romain Briandet,Julie Laurent,Laurent Beney,Pierre Lapaquette,Jean Guzzo,Aurélie Rieu