鞘内注射右美托咪定对神经性疼痛大鼠行为能力、镇痛效果及蛋白激酶C的影响
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Influence of intrathecal injection with dexmedetomidine on the behavioral ability and analgesic effects on rats with neuropathic pain and expression of protein kinase C in the spinal dorsal horn
背景与目的
本研究旨在探讨鞘内注射右美托咪定对慢性神经病理性疼痛大鼠模型的行为能力和镇痛作用的影响及蛋白激酶C（PKC）在脊髓背角中的表达在右美托咪定的镇痛机制中所起的作用。
方 法
35只Sprague Dawley大鼠，随机选择5只大鼠作为空白对照组，其余大鼠作为慢性压迫性损伤模型，分为模型组和观察组（每组15只）。观察组鞘内注射10ml右美托咪定，模型组注射等量生理盐水。通过累积和运动功能评分评估行为能力。此外，应用机械退缩阈值（MWT）和热退缩潜伏期（TWL）来检测疼痛阈值。免疫组织化学染色法检测PKC阳性表达，Western blot检测PKC定量表达水平。
结 果
启动后，模型和观察组的即时累积和运动功能评分增加，而MWT和TWL的值下降，模型和观察组脊髓背角PKC的阳性表达显着增加，定量表达水平也呈上升趋势。随着时间的推移，观察组的阳性和定量表达水平逐渐降低。
结 论
鞘内注射右美托咪定可以改善慢性神经痛大鼠的行为能力并降低疼痛程度，这可能与抑制脊髓背角PKC的表达有关。
原始文献摘要
XIAONING LI and WEI ZHANG；Influence of intrathecal injection with dexmedetomidine on the behavioral ability and analgesic effects on rats with neuropathic pain and expression of protein kinase C in the spinal dorsal horn；EXPERIMENTAL AND THERAPEUTIC MEDICINE 16: 3835-3840, 2018.
Background:
This study aimed to investigate the influence of intrathecal injection with dexmedetomidine on the behavioral ability and analgesic effects of rat models with chronic neuropathic pain. It also discusses the role that the expression of protein kinase C (PKC) in the spinal dorsal horn plays in the analgesic mechanism of dexmedetomidine.
Methods:
A total of 35 Sprague Dawley rats were selected. Five rats were randomly chosen as the blank control group and the rest were prepared as models with chronic constriction injury, which were divided into the model and the observation group (n=15, each group). The observation group was injected with 10 ml dexmedetomidine intrathecally, while the model group was injected with an equivalent amount of saline. The behavioral abilities were evaluated by cumulative and motor function score. In addition, mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were applied for the detection of pain threshold. Immunohistochemistry staining method was used to test the positive expression of PKC, and western blot analysis was used for the detection of PKC quantitative expression levels.
Results:
After initiation, the instant cumulative and motor function score of the model and observation groups increased, while the values of MWT and TWL decreased. After initiation, the positive expression of PKC in the spinal dorsal horn of the model and observation groups rose dramatically, and the quantitative expression level was also on the rise. The positive and quantitative expression levels in the observation group reduced gradually over time.
Conclusions:
Thus, the intrathecal injection of dexmedetomidine may improve the behavioral ability of rats with chronic neuralgia and reduce the degree of pain, which may be associated with the inhibition of the expression of PKC in the spinal dorsal horn.
麻醉学文献进展分享
贵州医科大学高鸿教授课题组
编辑：王贵龙 审校：李华宇