关于回复审稿意见，之前我们推送过一些内容。目前大家可能更需要真实案例来进一步学习和巩固。因此，我们整理了Nature Conmmunication近期（3月11日）2篇论文的部分审稿意见，供大家参考（全文获取方式见文末）。
链接：https://www.nature.com/articles/s41467-021-21841-w
Review#2: 作者应当简短的讨论一下最近……等人的工作。
感谢这位审稿人的建议。最近的一项研究表明雷帕霉素治疗可以降低……而在我们的研究中，这种治疗并没有明显影响……我们注意到该研究里的雷帕霉素治疗远高于我们研究中使用的浓度，在这种高浓度下，该治疗可以……从而抑制……We thank this reviewer for the suggestin. A recent study showed rapamycin treatment can decrease……, whereas in our study rapamycin treatment did not obviously affect …… We noticed that the rapamycin concentration used in this study (25µM) was much higher than that used in our study .It is possible that rapamycin at such high concentrations can……and thereby suppress …….另一项研究表明，mTORC1调节……，然而该研究表明，雷帕霉素治疗甚至增加了……需要进一步的研究来澄清这些问题。Another previous study indicated that mTORC1 regulates ……. However, this study showedthat rapamycin treatment even increased……. Further studies are needed to clarify these questions.
We now discussed and cited these relevant publications.
Reviewer #1：新的结果发现……这与手稿的主要结论矛盾。这是作者需要彻底说明的。
感谢审稿人提出有洞察力的问题。我们承认，我们目前还不了解机制层面上的这种调节选择性，但是我们想指出，在概念层面上，这在生物系统中是相当普遍的。We thank the reviewer for asking this insightful question.We acknowledge that we currently do not understand this regulation selectivity on the mechanistic level, but want to point out that, at the conceptual level, this is quite common in biological systems.如图1所示，mTORC1接受来自不同刺激的信号输入……一旦被激活就能促进……。去除……都可以有效地抑制mTORC1的活性；然而，……不会导致……的抑制。例如，已有研究表明，……
As illustrated in rebuttal letter Fig. 1, mTORC1 receives signaling inputs from …… Once activated, mTORC1 functions to promote……. Removal of ……can all potently suppress mTORC1 activity ……; however, ……would not cause the suppression of ……. For example, it has been shown that ……
有几种机制可以解释这种情况：（1）……（2）……（3）……Several mechanisms can account for such a selectivity in signaling regulation……由于我们目前的研究不是为了了解……对……的不同影响，我们希望这位审稿人能同意，解决这些问题超出了本研究的范围。我们已经将这些讨论纳入了我们的手稿，并希望这将促进未来对这一有趣问题的研究。As our current study is not set out to understand the differential effects of…… on ……, we hope this reviewer will agree that addressing these questions is beyond the scope of this study. We have incorporated these discussions into our manuscript, and hope that this will stimulate future studies on this interesting question.Reviewer #4：该论文详细介绍了实验方法的许多方面……令人遗憾的是，还有更广泛的关于实验设计和结果展示的解释是缺乏的。
我们同意这位审稿人的观点，即方法部分应该包括详细的实验条件，这样其他研究人员就可以在未来复制我们的发现。根据这位审稿人的友好建议，我们提供了实验设计的流程图，希望这可以帮助读者广泛地理解我们的实验方法。之后的回复也会有更多细节。（用图表丰富实验细节，便于其他研究者进行重复实验）We agree with this reviewer that the Methods section should include detailed experimental conditions so that other researchers can replicate our findings in the future. Following the kind suggestion of this reviewer, we now provide a flowchart of our proteomic experimental design,which we hope can help the readers to understand broadly our experimental approach.Our response below will provide additional clarifications and answers to this reviewer’s questions.
链接：https://www.nature.com/articles/s41467-021-21300-6#Sec20
Reviewer #1
……通路分析相对缺乏信息……没有进行与……变化等相关的假设检验……不清楚这些描述性质的数据如何拓展我们现有的知识？
我们感谢审稿人的建议。我们对图4C进行了更新，并对段落……进行了大量编辑，以澄清我们不打算提供药物预测分析……We thank the Reviewer for the suggestions. We have updated Fig.4C and introduced substantial edits to the paragraph……to clarify that we do not intend to provide a drug prediction analysis……关于路径假设检验，我们更新了先前版本的手稿中包含的分析。我们进行了差异表达分析，随后对……进行了GSEA分析，以确定……之间的一致性模式。路径选择基于……In regard to pathway hypothesis testing, we have updated the analysis included in the previous version of the manuscript. We have performed differential expression analysis followed by GSEA for ……to identify concordance patterns among ……. The selected pathways were chosen based on ……正如审稿人所说，已有公开的……，我们在修订后的手稿中加入了对新研究的进一步参考。然而，这些大多数来自……，由于患者接受……，获得了耐药性特征。相比之下，早期……As the Reviewer indicated, there are publicly available cohorts of …… and we have included further references to new studies in the revised manuscript.However, the majority of these cohorts are from ……, which have already acquired resistance traits due to ……. In contrast, there is limited availability of early stage ……我们建立的PNPCa模型提供了……，它保留了……因此，它可以作为与晚期PCa疾病模型比较的参考点(早期)。我们现在已经在手稿中加入了一项新的分析，比较了……的基因表达谱。The PNPCa model that we have established represents ……, which retains ……. Thus it may serve as a reference point (early stage) for comparison with models of advanced PCa disease. We have now included a new analysis in the revised manuscript, comparing the gene expression profiles of……本期回复意见原文可在公众号后台回复“317意见”获取哦~大家还想看什么领域的审稿意见回复案例呀？在下方投个票吧
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