Plant J:福建农林许卫锋教授在G蛋白γ亚基qPE9-1参与根系伸长对磷的吸收分子机制研究中取得新进展
异三聚体G蛋白参与植物生长发育，而水稻G蛋白γ亚基qPE9-1在低磷条件下的响应机制尚不清楚。LP条件下，qPE9-1基因在水稻根系中表达显著。携带qPE9-1等位基因的水稻品种对LP的主根响应高于携带qPE9-1等位基因的水稻品种(qPE9-1等位基因突变体)。在LP条件下，与qPE9-1等位基因相比，转qPE9-1基因水稻的主根长度和磷浓度均显著增加。质膜(PM) H+- atp酶在qpe9 -1介导的LP应答中起重要作用。此外，OsGF14b，一种14-3-3蛋白，作为激活PM H+- atp酶促进根系延伸的关键成分，也参与了qPE9-1的介导。此外，携带qpe9-1等位基因的WYJ8中过表达OsGF14b部分增加了LP下的主根长度。使用R18肽(14-3-3蛋白抑制剂)，我们的结果表明，qPE9-1通过参与14-3-3蛋白，在LP条件下对主根伸长和H+外排具有重要作用。土壤干燥条件下，qPE9-1株系根鞘重、全磷含量和根鞘土壤Olsen-P浓度均高于qPE9-1株系。
这些结果表明，水稻植株G蛋白γ亚基qPE9-1通过参与14-3-3蛋白OsGF14b和PM H+- atp酶调控根系伸长对磷的吸收。
Heterotrimeric G protein is involved in plant growth and development, while the role of rice G protein γ subunit qPE9-1 in response to low-phosphorus (LP) conditions remains unclear. The gene expression of qPE9-1 was significantly induced in rice roots under LP conditions. Rice varieties carrying the qPE9-1 allele showed higher primary root response to LP than the varieties carrying the qpe9-1 allele (mutant of qPE9-1 allele). Transgenic rice plants with the qPE9-1 allele had a longer primary root length and P concentration than those with the qpe9-1 allele under LP conditions. The plasma membrane (PM) H+-ATPase was important for the qPE9-1-mediated response to LP. Further, OsGF14b, a 14-3-3 protein that acts as a key component in activating PM H+-ATPase for root elongation, is also involved in the qPE9-1 mediation. Moreover, the overexpression of OsGF14b in WYJ8 (carrying the qpe9-1 allele) partially increased primary root length under LP. Using R18 peptide (a 14-3-3 protein inhibitor), our results showed that qPE9-1 was important for primary root elongation and H+ efflux under LP conditions by involving the 14-3-3 protein. In addition, rhizosheath weight, total P content and the rhizosheath soil Olsen-P concentration of qPE9-1 lines were higher than those of qpe9-1 lines in soil drying under LP conditions. These results suggest that the G protein γ subunit qPE9-1 in rice plants modulates root elongation for phosphorus uptake by involving the 14-3-3 protein OsGF14b and PM H+-ATPase, which is required for rice P use.
https://doi.org/10.1111/tpj.15402
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