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外泌体是指包含了复杂 RNA 和蛋白质的小膜泡 (30-150nm),现今,其特指直径在40-100nm的盘状囊泡。1983年,外泌体首次于绵羊网织红细胞中被发现, 1987年Johnstone将其命名为“exosome”。

多种细胞在正常及病理状态下均可分泌外泌体。其主要来源于细胞内溶酶体微粒内陷形成的多囊泡体,经多囊泡体外膜与细胞膜融合后释放到胞外基质中 。

外泌体文献

Cell

A TRP Channel SensesLysoome Neutralization by Pathogens to Trigger Their Expulsion

Acquisitionof Phage Sensitivity by Bacteria through Exchange of Phage Receptors

Adipose Tissue MacrophageDerived Exosomal miRNAs Can Modulate InVivo and InVitro Insulin Sensitivity(xuebalib.com)

An Actin NetworkDispatches Ciliary GPCRs into Extracellular Vesicles to ModulateSignaling

Bacterial OuterMembrane Vesicles Mediate Cytosolic Localization of LPS and Caspase-11Activation

Cell-cell communication between malaria-infected red blood cells via exosome-like vesicles

CirculatingImmune Cells Mediate a Systemic RNAi-Based Adaptive Antiviral Response inDrosophila

Communicationby Extracellular Vesicles Where We Are and Where We Need to Go

Drosophilosophical Rethinking Adaptive Immunity in the Fly

DynamicRemodeling of Membrane Composition Drives Cell Cycle through Primary CiliaExcision

Exosomes mediatestromal mobilization of autocrine Wnt-PCP signaling in breast cancer cellmigration

ExosomeTransfer from Stromal to Breast Cancer Cells Regulates Therapy ResistancePathways.

Extracellular Vesicles from Trypanosoma brucei Mediate Virulence FactorTransfer and Cause Host Anemia

Fateof the transferrin receptor during maturation of sheep reticulocytes in vitro selective externalization of the receptor

In Vivo imagingreveals extracellular vesicle-mediated phenocopying of metastaticbehavior

Kicking Out Pathogens in Exosomes

Retrovirus-likeGag Protein Arc1 Binds RNA and Traffics across Synaptic Boutons

The HippoPathway Kinases LATS12 Suppress Cancer Immunity

The NeuronalGene Arc Encodes a Repurposed Retrotransposon Gag Protein that MediatesIntercellular RNA Transfer

UnshieldingExosomal RNA Unleashes Tumor Growth And Metastasis

Vesicles SpreadSusceptibility to Phages

Nature

Adipose-derived circulating miRNAs regulate gene expression inother tissues

Cancer Organ-seeking vesicles

Designedproteins induce the formation of nanocage-containing extracellularvesicles.

Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer

Exosomes facilitate therapeutic targeting of oncogenic KRAS inpancreatic cancer.

Extracellular Vesicles from Trypanosoma brucei Mediate Virulence FactorTransfer and Cause Host Anemia

Glypican-1 identifies cancer exosomes and detects earlypancreatic cancer.

Hypothalamic stem cells control ageing speed partly throughexosomal miRNAs.

Macrophages redirect phagocytosis by non-professionalphagocytes and influence inflammation.

Microenvironment-induced PTEN loss by exosomal microRNA primesbrain metastasis outgrowth

The ESCRT machinery regulates the secretion and long-rangeactivity of Hedgehog.

The oyster genome reveals stress adaptation and complexity ofshell formation.

The tumour trail left in blood.

Tumour exosome integrins determine organotropic metastasis

Science

Cell biology Unconventional secretion unconventional solutions(xuebalib.com)

Ceramide triggers budding of exosome vesicles into multivesicular endosomes.

Genemicrobiota interactions contribute to the pathogenesis of inflammatory bowel disease(xuebalib.com)

Malignant messengers(xuebalib.com)

Menacing exosomes spread cancer(xuebalib.com)

No ESCRTs for exosomes

Science-2014-Anastasiadou-1459-60

The ins and outs of exosomes.

Viruses transfer the antiviral second messenger cGAMP between cells(xuebalib.com)

外泌体相关分离纯化实验方法

ChIRP--Chromatin Isolation by RNA Purification (ChIRP)

Chromatin Isolation by RNA Purification (ChIRP)

Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR-A Feasible Liquid Biopsy Tool

Isolation and Characterization of RNA-Containing Exosomes

Isolation and Characterization of RNA-Containing Exosomes

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

PULL DOWN--Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

碘克沙醇梯度离心-exosome

exosome讲座ppt与语音

Exosome in liver disease Rose

Thery et al., Exosome Protocols

2014-Chen G-Angewandte

2016-JACS 陈刚

Au nanoparticles enhanced

Targeted Drug Delivery with Polymers and Magnetic Nanoparticles- Covalent and Noncovalent Approaches, Release Control, and Clinical Studies

Visualization of immediate immune responses to pioneer metastatic cells in the lung.

外泌体_为高效药物投递策略提供天然的内源性纳米载体

外泌体与肿瘤转移

外泌体综述

细胞自噬在感染,炎症以及免疫中的作用

JCI exosome

Extracellular vesicle isolation andcharacterization toward clinical application

The biology and function of exosomes in cancer

Versatile roles of extracellularvesicles in cancer

Dendritic cell-derived exosomes forcancer therapy

Extracellular vesicles masters ofintercellular communication and potential clinical interventions

Regulation of chronic inflammatory andimmune processes by extracellular vesicles

Extracellular vesicles and infectiousdiseases new complexity to an old story

Exosomes and tumor-mediated immunesuppression

Extracellular vesicles andintercellular communication within the nervous system

Exosomes in stroke pathogenesis andtherapy

Prostasomes as a source of diagnosticbiomarkers for prostate cancer


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