南方医科大学：二氧化硅纳米颗粒对小鼠的肠道毒性 | 热心肠日报
介孔二氧化硅纳米颗粒对肠道的亚急性毒性及其潜在机制研究
10.1016/j.jhazmat.2020.124502
11-13, Article
Abstract & Authors:展开
Abstract:收起
The biological safety of mesoporous silica nanoparticles (MSNs) has gradually attracted attention. However, few studies of their toxicity to the intestine and mechanism are available. In this study, their primary structures were characterized, and their subacute toxicity to mice was investigated. After 2 weeks of intragastric administration of MSNs, they significantly enhanced serum ALP, ALT, AST and TNF-α levels and caused infiltration of inflammatory cells in the spleen and intestines. MSNs induced intestinal oxidative stress and colonic epithelial cell apoptosis in mice. Intestinal epithelial cells exhibited mitochondrial ridge rupture and membrane potential decrease after MSN treatment. Additionally, MSNs increased ROS and NLRP3 levels and inhibited expression of the autophagy proteins LC3-II and Beclin1. MSNs significantly changed the intestinal flora diversity in mice, especially for harmful bacteria, leading to intestinal microecology imbalance. Meanwhile, MSNs influenced the expression of metabolites, which were involved in a range of metabolic pathways, including pyrimidine metabolism, central carbon metabolism in cancer, protein digestion and absorption, mineral absorption, ABC transport and purine metabolism. These results indicated that the subacute toxicity of mesoporous silicon was mainly caused by intestinal damage. Thus, our research provides additional evidence about the safe dosage of MSNs in the clinical and food industries.
First Authors:
Yu-Di Deng
Correspondence Authors:
Xing-Fen Yang,Wen-Zhen Liao
All Authors:
Yu-Di Deng,Xu-Dong Zhang,Xu-Shan Yang,Zhen-Lie Huang,Xi Wei,Xing-Fen Yang,Wen-Zhen Liao