空间记忆相关蛋白的蛋白质组学研究

C57BL/6J小鼠在评价空间记忆的多T迷宫(MTM)和Barnes迷宫(BM)中表现不同, 本研究拟寻找导致这种行为差异的海马蛋白. 应用双向凝胶电泳结合质谱鉴定和数据库检索, 分析比较经两种迷宫训练测试后小鼠海马蛋白水平的不同, 发现经过BM和MTM训练的小鼠有29种蛋白表达存在明显差异. 其中, 在BM训练组中5种蛋白表达上调, 而在MTM组中24种蛋白表达上调. 与空间记忆相关的蛋白按功能可分为如下6类: (1)能量代谢相关蛋白; (2)细胞骨架相关蛋白; (3)分子伴侣; (4)神经发育相关蛋白; (5)转录因子和蛋白合成相关蛋白; (6)信号转导蛋白. 本研究结果丰富了空间记忆的机制, 对于神经科学的进一步发展具有启发意义.

关键词: Barnes迷宫,多T迷宫,蛋白质组学,空间记忆,质谱

C57BL/6J mice performed differently in Multiple T Maze (MTM) and Barnes Maze (BM) which were used for evaluation of spatial memory. This promoted us to explore proteins related to this cognitive difference. Hippocampal proteins from C57BL/6J mice after acquisition phase and probe trials in two mazes were extracted and subjected to 2D gel electrophoresis. Protein quantification was analyzed and differentially expressed spots were identified using mass spectrometry and MASCOT searching. Twenty-nine hippocampal proteins from C57BL/6J mice after two maze experiments were significantly different. Expressions of 5 proteins in hippocampus of C57BL/6J mice after BM experiment increased significantly. Twenty-four hippocampal proteins showed significantly increased expression in C57BL/6J mice after MTM experiment. These identified proteins linked to spatial memory could be divided into 6 categories according to their functions: (1) energy metabolism; (2) cytoskeleton; (3) chaperone; (4) neurogenesis; (5) transcription factor and protein synthesis; (6) signal transduction. The result enriched the content of spatial memory mechanism and could promote the development of neuroscience.

Key words: barnes maze,multiple T maze,proteomics,spatial memory,mass spectrometry

引用此文

郑君芳, 孙超渊, 孙丽翠, 杨晓梅, 王小柱, 黄艳, 李洋, 贺俊崎. 空间记忆相关蛋白的蛋白质组学研究[J]. 化学学报, 2010, 68(10): 996-1002.

ZHENG Jun-Fang, SUN Chao-Yuan, SUN Li-Cui, YANG Xiao-Mei, WANG Xiao-Zhu, HUANG Yan, LI Xiang, HE Dun-Qi. A Proteomic Survey of Spatial Memory Related Proteins in Mice[J]. Acta Chimica Sinica, 2010, 68(10): 996-1002.