PrimerBank Search Result

PrimerBankThe following matches are found for NCBI Gene ID (Mouse): "12064"Gene Descriptions:NCBI GeneID12064GenBank AccessionNM_001048141NCBI Protein AccessionNP_001041606SpeciesMouseCoding DNA Length 750Gene Description Mus musculus brain derived neurotrophic factor (Bdnf), transcript variant 3, mRNA.Primer Pair 1(Click here for cDNA and amplicon sequence):PrimerBank ID 114326460c1Amplicon Size 80Sequence (5' -> 3') Length Tm LocationForward Primer TCATACTTCGGTTGCATGAAGG 22 60.4 31-52Reverse Primer ACACCTGGGTAGGCCAAGTT 20 63.0 110-91Primer Pair 2(Click here for cDNA and amplicon sequence):PrimerBank ID 114326460c2Amplicon Size 89Sequence (5' -> 3') Length Tm LocationForward Primer AGGTCTGACGACGACATCACT 21 62.6 162-182Reverse Primer CTTCGTTGGGCCGAACCTT 19 62.6 250-232Primer Pair 3(Click here for cDNA and amplicon sequence):PrimerBank ID 114326460c3Amplicon Size 101Sequence (5' -> 3') Length Tm LocationForward Primer TTACCTGGATGCCGCAAACAT 21 62.4 351-371Reverse Primer TGACCCACTCGCTAATACTGTC 22 61.2 451-430Gene Descriptions:NCBI GeneID12064GenBank AccessionNCBI Protein AccessionNP_001041604SpeciesMouseCoding DNA Length 750Gene Description Mus musculus brain derived neurotrophic factor (Bdnf), transcript variant 2, mRNA.Primer Pair 1(Click here for cDNA and amplicon sequence):Validation Results  (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data)PrimerBank ID 34328442a1Amplicon Size 137Sequence (5' -> 3') Length Tm LocationForward Primer TCATACTTCGGTTGCATGAAGG 22 60.4 31-52Reverse Primer AGACCTCTCGAACCTGCCC 19 63.0 167-149Submit your own primers to PrimerBank