New Phytol:糖反应生物钟调节因子bZIP63调节植物生长
● 对能源匮乏的调整对于确保增长和生存至关重要。在拟南芥(Arabidopsis thaliana)中,这个过程部分依赖于SnRK1对生物钟调节器bZIP63的磷酸化,SnRK1是对低能反应的关键介体。
● 我们研究了bZIP63基因突变对植物碳代谢和生长的影响。bZIP63突变体的表型、转录组和代谢组学分析结果促使我们研究淀粉积累模式以及参与淀粉降解和昼夜节律振荡器的基因表达。
● bZIP63突变在光-暗循环下损害生长,但在恒定光照下不起作用。生长的减少可能是由于临近夜晚时C的消耗加剧,这是由bZIP63突变体的淀粉加速降解引起的。bZIP63的昼夜表达模式由生物钟和能量水平决定,这可能决定了bZIP63突变体中生物钟和淀粉代谢基因的昼夜表达变化。
● 我们的结论是bZIP63在淀粉分解的代谢和昼夜节律控制之间构成了一个调节界面,以优化C的使用和植物生长。
● Adjustment to energy starvation is crucial to ensure growth and survival. In Arabidopsis thaliana (Arabidopsis), this process relies in part on the phosphorylation of the circadian clock regulator bZIP63 by SnRK1, a key mediator of responses to low energy.
● We investigated the effects of mutations in bZIP63 on plant carbon (C) metabolism and growth. Results from phenotypic, transcriptomic, and metabolomic analysis of bZIP63 mutants prompted us to investigate the starch accumulation pattern and the expression of genes involved in starch degradation and in the circadian oscillator.
● bZIP63 mutation impairs growth under light-dark cycles, but not under constant light. The reduced growth likely results from the accentuated C depletion towards the end of the night, which is caused by the accelerated starch degradation of bZIP63 mutants. The diel expression pattern of bZIP63 is dictated by both the circadian clock and energy levels, which could determine the changes in the circadian expression of clock and starch metabolic genes observed in bZIP63 mutants.
● We conclude that bZIP63 composes a regulatory interface between the metabolic and circadian control of starch breakdown to optimize C usage and plant growth.
https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.17518?af=R
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