Nature Genetics|光敏色素相互作用因子PIFs触发植物对环境敏感的染色质动力学

光受体与光敏色素相互作用因子(photochromeinterinteraction FACTORs, PIFs)之间的相互作用是植物感知并将环境线索整合到转录网络中的调节枢纽1,2。虽然组蛋白变异H2A。Z和组蛋白H3的乙酰化已经成为环境响应基因网络的调节因子，但这些表观基因组特征如何与PIF活性相结合目前尚不清楚。

利用快速和可逆的光介导PIF7亚核定位和磷酸化操作，我们同时检测了PIF7的dna结合特性，以及它对全基因组染色质动力学的影响。我们发现PIFs快速重塑H2A。Z和H3K9ac表观遗传景观对光质量变化的响应。此外，我们发现PIFs可以实现H2A。通过与EIN6增强子(EEN)的直接相互作用去除Z, EIN6增强子(拟南芥染色质重塑复合体中INO80亚基6亚基的同源物(Ies6))。

因此，我们描述了一种PIF-INO80调控模块，它是允许植物改变其生长轨迹以应对环境变化的中间步骤。

The interplay between light receptors and PHYTOCHROME-INTERACTING FACTORs (PIFs) serves as a regulatory hub that perceives and integrates environmental cues into transcriptional networks of plants1,2. Although occupancy of the histone variant H2A.Z and acetylation of histone H3 have emerged as regulators of environmentally responsive gene networks, how these epigenomic features interface with PIF activity is poorly understood3,4,5,6,7. By taking advantage of rapid and reversible light-mediated manipulation of PIF7 subnuclear localization and phosphorylation, we simultaneously assayed the DNA-binding properties of PIF7, as well as its impact on chromatin dynamics genome wide. We found that PIFs act rapidly to reshape the H2A.Z and H3K9ac epigenetic landscape in response to a change in light quality. Furthermore, we discovered that PIFs achieve H2A.Z removal through direct interaction with EIN6 ENHANCER (EEN), the Arabidopsis thaliana homolog of the chromatin remodeling complex subunit INO80 Subunit 6 (Ies6). Thus, we describe a PIF–INO80 regulatory module that is an intermediate step for allowing plants to change their growth trajectory in response to environmental changes.

DOI:10.1038/s41588-021-00882-3

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