The Crop Journal|四川农大王文明团队揭示水稻miR171b–SCL6 IIs模块控制稻瘟病抗性、产量和开花
微小 rna (microrna)是植物发育和多种胁迫反应的调节因子。这里我们证明水稻 miR171b-SCL6-IIs 模块调节稻瘟病抗性、产量和开花之间的平衡。
过表达 miR171b 的水稻植株(OX171b)抗稻瘟病性增强，防御反应增强，抽穗后期，而阻断 miR171b 在水稻中的表达，导致水稻对稻瘟病的易感性增强，防御反应减弱，抽穗早期。miR171b 基因过表达或沉默均显著影响株高和每穗结实粒数(结实率) ，导致产量下降。miR171b 作用于 SCL6-IIa、 SCL6-IIb 和 SCL6-IIc，其表达在 OX171b 中受到抑制，在 MIM171中增加。SCL6-IIa、 SCL6-IIb 和 SCL6-IIc 的突变体均表现出与 OX171b 相似的表型，包括抗稻瘟病性显著增强，产量略有下降，开花时间推迟。miR171b 在营养生长阶段叶片中的含量逐渐增加，在生殖生长阶段叶片中的含量逐渐减少，而 SCL6-IIs 表现出相反的表达模式。这些结果表明 miR171b 的表达在水稻生育期具有时间和空间依赖性，通过 SCL6-IIs 调节稻瘟病抗性、产量和开花之间的平衡，miR171b 的合理积累对水稻生长发育至关重要。
MicroRNAs (miRNAs) act as regulators of plant development and multiple stress responses. Here we demonstrate that the rice miR171b-SCL6-IIs module regulates the balance between blast resistance, grain yield, and flowering. miR171b-overexpressing rice plants (OX171b) displayed increased rice blast resistance accompanied with enhanced defense responses and late heading, whereas blocking miR171b expression in rice (MIM171) led to greater susceptibility to blast disease, associated with compromised defense responses and early heading. Either overexpressing or silencing of miR171b significantly affected plant height and number of filled seeds per panicle (seed-setting rate), resulting in decreased grain yield. miR171b targets SCL6-IIa, SCL6-IIb, and SCL6-IIc, whose expression was suppressed in OX171b but increased in MIM171. Mutants of SCL6-IIa, SCL6-IIb, and SCL6-IIc all displayed phenotypes like that of OX171b, including markedly increased blast disease resistance, slightly decreased grain yield, and delayed flowering. Amounts of miR171b increased gradually in leaves during the vegetative stage but decreased gradually in panicles during the reproductive stage, whereas SCL6-IIs displayed the reverse expression pattern. Together, these results suggest that the expression of miR171b was time- and space-dependent during the rice growth period and regulated the balance between rice blast disease resistance, grain yield, and flowering via SCL6-IIs, and that appropriate accumulation of miR171b is essential for rice development.
https://www.sciencedirect.com/science/article/pii/S2214514121001094?dgcid=rss_sd_all#!
文章来源原创，转载请注明来源。
温馨提示：
为方便PaperRSS粉丝们科研、就业等话题交流。我们根据10多个专业方向（植物、医学、药学、人工智能、化学、物理、财经管理、体育等），特建立了30个国内外博士交流群。群成员来源欧美、日韩、新加坡、清华北大、中科院等全球名校。