四川农业大学：限时进食可改善小鼠的生殖功能 | 热心肠日报

限时进食通过肝脏FGF21改善雌鼠的生殖功能

10.1002/ctm2.195

10-04, Article

Abstract & Authors:展开

Abstract:收起
Background: There has been a significant increase, to epidemic levels, of obese and overweight women of reproductive age, causing impairments to reproductive health. Time‐restricted feeding (TRF) including isocaloric intake has shown to be preventive of obesity‐related disorders. However, its therapeutic ability to improve the reproductive function of female remains largely unknown.
Methods: Here, we investigated the ability of TRF to improve the reproductive function in wild‐type and liver‐specific FGF21 knockout female mice. To study fertility, a continuous and a short‐term fertility test, gonadotropin releasing‐hormone (GnRH), and Kisspeptin test were performed. Immortalized GnRH neuron was used to examine the direct role of liver fibroblast growth factor 21 (FGF21) on GnRH secretion.
Results: We found that TRF rescues female mice from bodyweight gain and glucose intolerance, as well as ovarian follicle loss and dysfunction of estrus cyclicity induced by high‐fat diet. Furthermore, the beneficial effects of the TRF regimen on the reproductive performance were also observed in mice fed both chow and high‐fat diet. However, those beneficial effects of TRF on metabolism and reproduction were absent in liver‐specific FGF21 knockout mice. In vitro, FGF21 directly acted on GnRH neurons to modulate GnRH secretion via extracellular regulated protein kinases (ERK1/2) pathway.
Conclusions: Overall, time‐restricted feeding improves the reproductive function of female mice and liver FGF21 signaling plays a key role in GnRH neuron activity in female mice.

First Authors:
Lun Hua,Bin Feng

Correspondence Authors:
De Wu,Yong Zhuo

All Authors:
Lun Hua,Bin Feng,Liansu Huang,Jing Li,Ting Luo,Xuemei Jiang,Xingfa Han,Lianqiang Che,Shengyu Xu,Yan Lin,Zhengfeng Fang,De Wu,Yong Zhuo