J EXP BOT|APETALA2转录因子LsAP2调节生菜的种子形状

种子是植物繁殖和传播的主要媒介。包括APETALA2（AP2）在内的许多转录因子在植物种子发育过程中起着至关重要的作用。然而，生菜种子发育所必需的基因和种子发育过程中的调控网络尚不清楚。本研究，我们鉴定了一个生菜AP2（LsAP2）基因，它在种子发育的早期阶段高度表达。利用CRISPR/Cas9系统获得LsAP2基因敲除植株，研究LsAP2的生物学功能。与野生型相比，Lsap2突变体植株的种子长度增加，宽度减小，并在种子顶端形成一个延伸的顶端。在进一步研究Lsap2突变体的种子结构特征后，我们提出了Lsap2在种子传播中的新功能。此外，我们确定了LsAP2的几个相互作用者。结果表明，LsAP2直接与短梗生菜同源物（LsBP）相互作用，促进LsBP的表达。转录组分析显示LsAP2可能参与油菜素甾体的生物合成和信号传导途径。综上所述，我们的数据表明，LsAP2在莴苣种子形状的调节中有着重要的作用。

Seeds are major vehicles of propagation and dispersal in plants. A number of transcription factors, including APETALA2 (AP2), play crucial roles during the seed development process in various plant species. However, genes essential for seed development and the regulatory networks during seed development remain unclear in lettuce. Here, we identified a lettuce AP2 (LsAP2) gene that was highly expressed at the early stages of seed development. LsAP2 knockout plants obtained by the CRISPR/Cas9 system were used to explore the biological function of LsAP2. Compared with wild-type, the seeds of the Lsap2 mutant plants had increased length and decreased width, and developed an extended tip at the seed top. After further investigating the seed structural characteristics of Lsap2 mutant plants, we proposed a new function of LsAP2 in seed dispersal. Moreover, we identified several interactors of LsAP2. Our results showed that LsAP2 directly interacted with the lettuce homolog of BREVIPEDICELLUS (LsBP) and promoted the expression of LsBP. Transcriptome analysis revealed that LsAP2 might also be involved in brassinosteroid biosynthesis and signaling pathways. Taken together, our data indicate that LsAP2 has a significant function in regulating seed shape in lettuce.

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