茶树挥发物如何响应茶绿叶蝉的采食
第一作者
Huifan Liu
第一单位
仲恺农业工程学院
通讯作者
Qin Wang
Abstract
背景+问题：Oriental Beauty is a high-grade, oolong tea with a pronounced honey-like aroma and rich ripe fruit flavor that develops primarily as a result of the infestation of the fresh leaves by tea green leafhoppers (Empoasca (Matsumurasca) onukii Matsuda).
主要研究：Here, we used PacBio Iso-Seq and RNA-seq analyses to determine the full-length transcripts and gene expression profiles of fresh tea leaves in response to E. (M.) onukii herbivory. We investigated the relationship between RNA-seq, tea metabolites, and aroma response mechanisms in leaves infested by leafhoppers.
结果1-DEG and AS: We found 3644 differentially expressed genes, of which 2552 were up- and 1092 were down-regulated. A total of 49,913 alternative splicing events were predicted, including 324 differential AS events. Moreover, 3105 differentially expressed transcripts were also identified, of which 2295 were up- and 810 were down-regulated.
结果2-DEG and DEM：The characterization of expression patterns of the key gene transcript isoforms involved in the aroma formation pathways identified 130 differentially expressed metabolites, 97 of which were up- and 33 were down-regulated. Two key aroma compounds (phenylacetaldehyde and 4-hydroxybenzaldehyde) were highly correlated with genes of the aroma formation pathways.
结论：Our results revealed that pre-mRNA AS plays a crucial role in the metabolic regulation surrounding aroma formation under leafhopper herbivory in tea plants.
摘 要
东方美人茶是一种高档的乌龙茶，具有明显的蜂蜜样香气和丰富的成熟水果香气，其主要原因是茶绿叶蝉对新鲜叶片的侵染。在这里，我们使用PacBio Iso-Seq和RNA-seq分析来确定新鲜茶叶响应绿叶蝉的全长转录本和基因表达谱。我们调查了叶蝉侵染的叶片中RNA-seq，茶代谢产物和香气响应机制之间的关系。我们发现了3644个差异表达基因。总共预测了49,913个替代剪接事件，包括324个差异表达AS事件。此外，还鉴定了3105个差异表达的转录本，其中2295个上调而810个下调。鉴定出130种差异表达的代谢产物，其中97种上调而33种下调。两种关键的香气化合物（苯乙醛和4-羟基苯甲醛）与香气形成途径的基因高度相关。研究结果表明，AS在茶树叶蝉采食后香气形成的代谢调控中起着至关重要的作用。
doi: https://doi.org/10.1007/s00299-021-02674-9
Journal: Plant Cell Reports
Published：22 February 2021
END
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