Plant J|中国农大黄瓜团队在乙烯途径调控黄瓜表皮毛研究取得新进展
葫芦科植物的果毛被认为是许多亚洲国家的市场偏好,是黄瓜商品化生产和育种的关键品种选择决定因素。然而,我们对黄瓜毛状体的发生和发展过程的了解仍然是有限的。
我们发现黄瓜细小分支毛(tbh)基因在多细胞毛状体中优先表达。在 tbh 突变体中过量表达 cstbh 可以恢复毛状体的表型,提高雌花百分比,而在野生型植株中沉默 cstbh 则导致发育不良,雌花率较低。
此外,我们还证明 cstbh 能直接与黄瓜1- 氨基环丙烷 -1- 羧酸合成酶(csacs)基因启动子结合并调控其表达,从而影响多细胞毛发育、乙烯积累和性表达。
两个黄瓜 acs 突变体具有不同的毛状体形态和性别变异与他们的近等基因系进一步支持我们的发现。
我们的研究为 cstbh 通过乙烯途径调节多细胞毛发生长和性表达的分子机制提供了新的信息。
The fruit trichomes of Cucurbitaceae are considered the market preference among many Asian countries and have been a key determinant of cucumber cultivar selection for commercial production and breeding. However, our understanding of the initiation and development processes of cucumber trichomes is still limited. Here, we found that the cucumber TINY BRANCHED HAIR (TBH) gene was preferentially expressed in multicellular trichomes. Overexpressing CsTBH in tbh mutants restored the trichome phenotype and increased the percentage of female flowers, whereas silencing CsTBH in wild‐type plants resulted in stunted trichomes with a lower rate of female flowers. Furthermore, we provided evidence that CsTBH can directly bind to the promoters of cucumber 1‐Aminocyclopropane‐1‐Carboxylate Synthase (CsACS) genes and regulate their expression, which affects multicellular trichome development, ethylene accumulation and sex expression. Two cucumber acs mutants with different trichome morphology and sex morphs compared with their near‐isogenic line further supports our findings. Collectively, our study provides new information on the molecular mechanism of CsTBH in regulating multicellular trichome development and sex expression through an ethylene pathway.
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