Methods Mol Biol.|一种稳定转化水稻种子的快速方法

植物转化技术为基础科学和转化研究提供了大量的机会。几种农杆菌介导的植物转化方法可用于水稻愈伤组织的诱导和再生。经常使用的转化程序需要时间和熟练的劳动力，并且受到组织再生能力的限制。本文以pCAMBIA-amiR820为模型构建，描述了一种简单、健壮、与组织培养无关的水稻种子转化方法。从转化种子中获得的植株在抗生素培养基上进行筛选，并通过分子技术进行转基因整合和表达检测。由此产生的转基因幼苗包括稳定的转化体和嵌合体的混合物；然而,通过该方法第一代种子含有稳定整合的转基因。

PMID: 33471324 DOI: 10.1007/978-1-0716-1068-8_4

Plant transformation technology offers ample opportunities for basic scientific and translational research. Several Agrobacterium-mediated plant transformation protocols are available, for transforming rice, through callus initiation and regeneration. The regularly used transformation procedures require time and skilled labor and are limited by the regeneration capabilities of the tissue. Here we describe a simple, robust and tissue culture-independent method for transformation of rice seeds using pCAMBIA-amiR820 as model construct. Plants obtained from the transformed seeds were selected on antibiotic media and tested for transgene integration and expression by molecular techniques. The transgenic seedlings thus produced include a mix of stable transformants and chimeras; however the first generation seeds contained stably integrated transgene.

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