茶树茎尖和根中不同代谢特征的分子基础：MYB促进茶氨酸合成的表征

Molecular Basis of the Distinct Metabolic Features in Shoot Tips and Roots of Tea Plants (Camellia sinensis): Characterization of MYB Regulator for Root Theanine Synthesis

第一作者

Yanrui Zhang

第一单位

安农大

通讯作者

Jian Zhao

Abstract

背景+问题：The physiological and metabolic differences between shoot tips and roots of tea plants are significant, and understanding them is required for improvement of tea quality and plant growth.

主要研究：A high-quality full-length transcriptome sequencing on tea plant roots and shoot tips by PacBio SMRTtechnology was done to gain a further understanding.

结果1：Approximately 160699 and 166120 full-length transcripts were recovered in roots and shoots, respectively, including 31232 and 41068 novel isoforms and 16960 and 26029 alternative splicing (AS) isoforms. These supported 21699 full-length reads and 31232 and 41068 novel transcripts from root and shoot, respectively, including 1679 long noncoding RNAs (lncRNAs) and 2772 fusion transcripts, which significantly upgrade the Camellia sinensis genome annotation. The respective 6475 and 6981 transcripts in roots and shoots differ in 3′-untranslated regions. Meanwhile, extensive analyses of novel transcripts, ASs, and lncRNAs also revealed a large number of ASs and lincRNAs closely related to the regulation of characteristic secondary metabolites including catechins, theanine, and caffeine.

结果2：Finally, a root-specific CsMYB6 was characterized to regulate theanine biosynthesis by genetic and molecular analyses. CsMYB6 directly bound to and activate the promoter of theanine synthetase gene (CsTSI).

结论：The study lays a foundation for the further investigation of metabolic genomics and regulation in tea plants.

摘  要

茶树的梢和根之间的生理和代谢差异非常明显，因此了解它们对于改善茶的质量和植物的生长是必需的。通过PacBio SMRT技术对茶树根和枝条进行了高质量的全长转录组测序，以进一步了解。在根和芽中分别鉴定了大约160699和166120个全长转录本，包括31232和41068新的isform以及16960和26029可变剪接（AS）isform。这些分别支持来自根和芽的21699个全长读段和31232和41068个新的转录本，包括1679个长非编码RNA（lncRNA）和2772个融合转录本，这些显着提升了茶树的基因组注释。根和芽中的6475和6981各自的转录本在3'非翻译区中不同。同时，对新的转录本，AS和lncRNA的广泛分析还发现，大量的AS和lincRNA与儿茶素，茶氨酸和咖啡因等特征性次生代谢产物的调节密切相关。最后，通过遗传和分子分析表征了根特异性CsMYB6调节茶氨酸的生物合成。CsMYB6直接结合并激活茶氨酸合成酶基因（CsTSI）的启动子。

DOI: https://doi.org/10.1021/acs.jafc.0c07572

Journal: Journal of Agricultural and Food Chemistry

Impact Factor：4.192

Published date: March 15, 2021

END

三月

16

科学技术史上的今天

1926年3月16日，美国科学家罗伯特·戈达德在马萨诸塞州的奥巴恩发射了世界上第一枚液体燃料火箭。火箭长3.04米，用汽油和液态氧混合作为燃料，飞行2.5秒，达12米高，56米远。虽然这些数字在现在看来并不惊人，但却具有划时代的意义。

1978年3月16日，利比里亚油轮“阿莫科·卡迪兹号”在英国波特赛尔搁浅，船上22万吨原油倾流大海，污染海滩160余公里。

Where there is a will, there is a way!