急性心肌梗死后循环血中microRNA-208b的初步研究:对6个月生存率的影响。

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A Preliminary Study of microRNA-208b after Acute Myocardial Infarction: Impact on 6-Month Survival

  摘 要  

1

背景与目的

3

结果

2

方法

4

结论

背景与目的：miRNA有助于多种基本生物进程，包括发育，增殖，分化和凋亡。循环microRNA非常稳定，并且已显露出作为心血管疾病生物标志物的潜力。急性心肌梗死（AMI）患者的血液中microRNA-208b表达增加，并且已经被提议作为早期诊断的生物标志物。在这项初步研究中，我们研究了循环miR-208b作为AMI患者6个月生存率预后的生物标志物潜力。

1

方法：收集来自21名患者及8名年龄和性别相匹配的健康成年人的血浆样品，并使用RT-PCR技术检测循环血中miR-208b的表达水平。

结果：健康对照组中miR-208b水平较高（9.6倍;P≤0.05）。在AMI患者中，幸存组循环血中miR-208b的表达水平较非存活组显著降低（倍数变化= 6.51和14.1，分别为P≤0.05）。Kaplan-Meier曲线显示，AMI患者的6个月生存率明显较高，miR-208b的相对表达低于12.38。循环血中miR-208b的相对表达危险比（HR）（<12.38为参考）是5.08（95％CI：1.13-22.82; P = 0.03）。

结论：我们的研究结果表明，miR-208b的表达升高与AMI患者的长期存活率降低相关。这些试验数据表明还需要进行大量的随访研究，以确认miR-208b是否可作为AMI后6个月生存率的预测因子。

    原始文献来源   

Jiang B¹, Liu Y¹, Liang P²,et al.microRNA-126a-5p enhances myocardial ischemia-reperfusion injury through suppressing Hspb8 expression[J].Oncotarget, Oct 7，2017;8(55):94172-94187

Introduction miRNAs contribute to a variety of essential biological processes including development, proliferation, differentiation, and apoptosis. Circulating microRNAs are very stable and have shown potential as biomarkers of cardiovascular disease. microRNA-208b expression was increased in the blood of patients with acute myocardial infarction (AMI) and has been proposed as a biomarker for early diagnosis. In this pilot study, we investigate the potential of circulating miR-208b as a prognostic biomarker of 6-month survival in AMI patients.

Methods Plasma samples from 21 patients and 8 age- and gender-matched healthy adults were collected, and circulating levels of miR-208b were detected using quantitative real-time PCR.

Results miR-208b levels were higher in healthy control subjects (9.6-fold; P ≤ 0.05). Within the AMI patients, the levels of miR-208b were significantly lower in the survivor versus nonsurvivor group (fold change = 6.51 and 14.1, resp.; P ≤ 0.05). The Kaplan-Meier curve revealed that the 6-month survival time was significantly higher among AMI patients with a relative expression of miR-208b lower than 12.38. The hazard ratio (HR) for the relative expression of miR-208b (<12.38 was the reference) was 5.08 (95% CI: 1.13-22.82; P = 0.03).

Conclusion Our results showed that elevated miR-208b expression was associated with reduced long-term survival in AMI patients. These pilot data indicate the need for a large follow-up study to confirm whether miR-208b can be used as a predictor of 6-month survival time after AMI.

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