VSVG慢病毒感染不同细胞系和原代细胞的相对效率

Cell type相对感染效率（2）Cell lines(human)HEK293embryonic kidney cells++++TE671rhabdomyosarcoma cells++++HeLacervical adenocarcinoma++++HepG2hepatoma cells++++IB3-1CF bronchial epithelial cells+++K562chronic myelogenous leukemia++++U937myeloid leukemia cells++++A20B cell lymphoma+EBVblastsEBV transformed B lymphoma+++(mouse)Rencarenal cell carcinoma++++CT26colon carcinoma++++NIH3T3fibroblasts+++BaF3pre-B lymphoma+++TF1proerythro blastic leukemia cells++++DC2.4immortalized immature DC++/+++P19embryonic carcinoma+++Primarycells(human)HUVECumbilical vein endothelial cells+++CD34+cellshematopoietic stem/progenitor cells++/+++monocytesmyeloid cells++DCimmature dendritic cells+++Tcellslymphocytes+++Bcellslymphocytes+(other species)ESCmouse embryonic stem cells+++neuronrat neuronal cells+++liverstemcellsrat oval cells+++liverprimary rat hepatocytes++RPErat retinal pigment epithelial cells++++rodchicken eyerod cells++bonemarrowmouse BM mixed population+++HSCmouse hematopoietic stem/progenitor cells++（1）上表中的原代细胞感染效率和部分细胞系感染效率基于不同文献中的实验结果。由于采用了不同报告基因和载体，感染效率可能有很大差别。表中的感染效率仅供参考。（2）相对感染效率定义：++++=MOI为1-5时有大于50%细胞感染；+++=MOI为5-10时有大于50%细胞感染；++=MOI为10-20时有大于30%细胞感染；+=MOI大于20时有1-20%细胞感染。